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Cellular kinetic 24 hs and 48 hs.
Once the optimum formulation and dosage were determined
according to inhibitory action of growth up in BW cellular lines
in 24 hours, it came the experimentation the kinetic in 48 hours.
In TABLE 1: "Growth up' inhibition of BW cellular line in 24
hours." They're presented the results of the 2 formulations
(PF1 y PF3), as a percentage of growth up's inhibition of BW cellular
lines in 24 hours, that in first phase of experimentation showed
significant differences in reference to inhibition percentages of
cellular growth up.
| Dilution |
PF1 |
PF3 |
|
Stimulation Rate (%) |
Inhibition (%)
|
Stimulation Rate (%) |
Inhibition
(%) |
| 1/10 |
0.15 |
84.7 |
0.14 |
85.3 |
| 1/25 |
0.28 |
71.4 |
0.26 |
73.6 |
| 1/50 |
0.52 |
47.9 |
0.53 |
47.0 |
| 1/100 |
0.76 |
23.7 |
0.58 |
41.8 |
| 1/200 |
0.89 |
10.9 |
0.68 |
31.7 |
| 1/400 |
0.95 |
4.3 |
0.77 |
22.9 |
TABLE 1: Growth up's inhibition in
BW cellular line in 24 hs.
Again as we see in TABLE 1: "Inhibition of BW cellular line's
growth up in 24hs.", to bigger dilutions the following percentages
of inhibition were decrecent, resulting the main efficacy with PF3
dilution.
In TABLE 2: "Inhibition of BW cellular line's growth up in
48 hs.", the results are expressed in percentages of inhibition
of growth up in BW cellular lines in 48 hours. Once more were included
PF1 and PF3 formulations as these one in the first phase of experimentation
showed relevant differences in reference to the percentages of inhibition
of cellular's growth up, trying to discharge the possibility of
a different conduct at the 48 hours.
|
Dilution |
PF1 |
PF3 |
|
Stimulation's Rate (%) |
Inhibition (%)
|
Stimulation's
Rate (%) |
Inhibition
(%) |
|
1/10 |
0.001 |
99.8 |
0.001 |
99.87 |
|
1/25 |
0.007 |
99.2 |
0.002 |
99.7 |
|
1/50 |
0.16 |
84.0 |
0.028 |
97.0 |
|
1/100 |
0.68 |
31.57 |
0.24 |
75.0 |
|
1/200 |
0.87 |
13.0 |
0.65 |
31.0 |
|
1/400 |
0.92 |
7.8 |
0.72 |
28.2 |
TABLE 2: Inhibition
of BW cellular line's growth up in 48hs.
As a first conclusion in both formulations after 48 hours the inhibition
effect at therapeutic dose was bigger than the one in 24 hours,
that shows an accumulation effect (accumulation kinetic) to improve
the wished objective that is an non proliferation effect. Also at
48 hours, the bigger inhibition of BW cellular line's growth up
in absolutes terms at the therapeutic dose resulted in PF3 formulation
This way cellular kinetic is braked avoiding tumor's growth up.
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